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Abstract

Pollen germination and viability of two species viz., Jasminum sambuc (L.) Ait., and Nycanthes arbor-tristis L. of the family Oleaceae were examined . Viability was determined on the basis of germination ability of pollen on sucrose medium with boric acid solution up to 48 weeks. Stored pollen were germinated under different conditions (+4°C), (-20°C, -30°C), (-60°C), vacuum chamber and organic solvents. Pollen stored at low temperature -60°C showed better germination as compared to pollen stored at +4°C and fresh. Among two species Jasminum sambac (L.) Ait. showed much better germination percentage at -60°C ( 40.60% ) whereas, Nycanthes arbor-tristis L., showed 37.0% germination after 48 weeks of storage. Pollen stored over silica jel under vacuum of both the species showed good germination as compared to organic solvents.

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