PJB-2011-279
OPTIMIZATION OF CULTURAL CONDITIONS FOR THE BIOSYNTHESIS OF LIPASES BY PENICILLIUM CHRYSOGENUM (MBL 22) THROUGH SOLID STATE FERMENTATION
TEHREEMA IFTIKHAR*1, MUBASHIR NIAZ1, ZAIB UN NISA1, ASMA TARIQ1, M.N.KHALID1 AND RUKHSANA JABEEN2
Abstract
The present study was conducted in the Laboratory of Biotechnology and Mycology, Department of Botany, GC University, Faisalabad with the objective to study the production of extracellular lipases by Penicillium chrysogenum through solid- state fermentation (SSF). Penicillium chrysogenum was screened for the extracellular lipase production. The maximum extracellular lipase activity was obtained at 28oC after 72 h by using 1 mL inoculum level. 15g of mixed substrates (Almond+ Soybean+ Sunflower) was optimized for maximum extracellular lipase production. Agricultural by products, nitrogen sources and carbon sources were also optimized for the maximum production of enzyme. Maximum production of the extracellular lipase (64.77±0.155a U/mL) was obtained when olive oil was used as additional oil. Tween 80 and yeast extract were optimized as the best additional carbon and nitrogen sources respectively.
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