PJB-2011-281
PROCESS OPTIMIZATION FOR A POTENT WILD AND MUTANT STRAIN OF ASPERGILLUS NIGER FOR BIOSYNTHESIS OF AMYLOGLUCOSIDSAE
SHAZIA MALIK1, TEHREEMA IFTIKHAR2*AND IKRAM UL HAQ1
Abstract
The present study is concerned with the selection of a potent strain of Aspergillus niger and optimization of the cultural conditions for the biosynthesis of amyloglucosidase. The cultural conditions were optimized for the enzyme production. Twenty percent (50/250ml flask) was found to be optimum volume of the medium. Optimum temperature was 30oC after 72 h of incubation, with the initial pH of the medium 5.0. 2% Starch with 1% glucose as an additional carbon source gave maximum amyloglucosidase production Addition of 0.3% ammonium sulphate in the fermentation medium increased the enzyme production while 2% spore inoculum showed best amyloglucosidase production.
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