PJB-2012-229
OSGSK3 IS A NOVEL GSK3/SHAGGY-LIKE GENE FROM ORYZA SATIVA L., INVOLVED IN ABIOTIC STRESS SIGNALING
XIAN-GUANG YANG1, WEI-HONG LIANG1, FEN LI1 AND WEN-SHI MA2*
Abstract
Plant glycogen synthase kinase3/shaggy-like (GSK-3-like) kinases are produced by a multigene family. Here, a novel full-length cDNA encoding rice glycogen synthase kinase3/shaggy-like (GSK-3-like) kinase was cloned for the first time using reverse transcription polymerase chain reaction (RT-PCR). In order to investigate the genomic organization of OsGSK3, southern hybridization was done using OsGSK3 cDNA as the probe, and genome DNA were digested by EcoR I and HindIII restriction enzyme separately. Results illustrated that there was only one copy of OsGSK3 in the rice genome. Studies evaluating changes in the plants over time showed that the accumulation of OsGSK3 in the rice cultivars Nipponbare (Oryza sativa L.subsp. japonica) was increased by salt stress (170 mM NaCl), mechanical injury, ABA hormone, cold (4°C) and drought. These results suggest that cell accumulate more OsGSK3 mRNA response to those abiotic stresses. This suggests that enhanced OsGSK3 expression may be related to abiotic stress response, and may play an important role in the stress signal transduction. In this way, OsGSK3 was shown to be a positive regulator and to have some part in rice tolerance to salt, mechanical injury, ABA hormone, cold, and drought.
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