PJB-2012-231
BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SOMACLONAL VARIANTS AND INDUCED MUTANTS OF POTATO (SOLANUM TUBEROSUM L.) CV. DESIREE
HUMERA AFRASIAB¹* AND JAVED IQBAL²
Abstract
In the present study, tissue culture and mutagenic techniques were applied to induce variations in potato (Solanum tuberosum L.) cultivar, Desiree. For obtaining somaclonal variants, internode-derived calli were incubated for 14-20 weeks in callus inducing medium. For mutation induction, ten week old, well proliferating calli were exposed to 5-50 Gy of gamma irradiation. Three variant lines (SV1, SV2 and SV3) and 6 gamma mutant lines (GM1, GM2, GM3, GM4 GM5 and GM6) were selected for further biochemical and molecular studies. In general an increase in total peroxidase activity in all the selected variants and mutants was observed. Same results were obtained when qualitative study was conducted on PAGE (polyacrylamide gel electrophoresis).Variation at isozymes and random amplified polymorphic DNA (RAPD) were used to detect variability and a total of 24 arbitrary sequence primers were evaluated. The RAPD primers produced 123 bands (88 polymorphic), whereas isozymes peroxidase produced 6 bands (5 polymorphic) showing genetic variation as compared to control. The current study demonstrates the production of useful variants both by tissue culture and gamma irradiation in potato and the use of isozymes and RAPD for assessing the genetic diversity among the variants and mutants.
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