PJB-2012-306
STUDY OF TOTAL SEED PROTEINS PATTERN OF SESAME (SESAMUM INDICUM L.) LANDRACES VIA SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECROPHORESIS (SDS-PAGE)
FAZAL AKBAR1, NAHIDA YOUSAF2, M. ASHIQ RABBANI2*, ZABTA KHAN SHINWARI1 AND M. SHAHID MASOOD2
Abstract
The sesame (Sesamum indicum L.) germplasm, comprising of 105 accessions was characterized for total seed storage proteins using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The germplasm was collected from diverse agro-ecological regions of Pakistan. To our information, no studies have yet been carried out in Pakistan on the genetic evaluation of sesame genotypes based on total seed protein. Total seed proteins were electrophoretically separated on 12% polyacrylamide gels by standard protocols. A total of 20 polypeptide bands were observed, of which 14 (70%) were polymorphic and 6 (30%) were monomorphic, with molecular weight ranging from 13.5 to 100 kDa. Six bands i.e., 7
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