PJB-2013-207
PROTOCOL OPTIMIZATION FOR EXTRACELLULAR LIPASE PRODUCTION BY TRICHOPHYTON SPP. (MBL 23) UNDER SOLID STATE FERMENTATION
MUBASHIR NIAZ1, TEHREEMA IFTIKHAR1,2*, FAIZA AKRAM1 AND MUSHMOOM NIAZ3
Abstract
The present study was conducted in the Laboratory of Mycology and Biotechnology, Department of Botany, Government College University, Faisalabad with the objective of process optimization for the production of extracellular lipases by Trichophyton sp. through solid-state fermentation (SSF) technique. Almond meal proved to be the best single substrate with enzyme production of 12.25±0.11U/mL, however, the production was further enhanced by manipulating the environmental variables like combined substrates, etc. Fifteen grams of mixed substrates (Almond+ Sunflower + Brassica) at 1:1:1 ratio was optimized for maximum extracellular lipase production (60.36 ± 0.10a U/mL). The extracellular lipase activity at 26oC after 48 h of incubation at diluent pH of 7 and by using 1 mL inoculum level was 68.65 ± 0.13a U/mL. Agricultural byproducts, nitrogen sources and carbon sources were also optimized for the maximum production of enzyme. Almond oil and Glucose were also found to be supportive carbohydrate & lipid additives to agro-wastes for hyperproduction of extracellular enzyme that improved the lipase production upto 73.34 ± 0.135a and 75.58 ± 0.083a U/mL, respectively. Maximum production of the extracellular lipase (76.42±0.036a U/mL) was obtained when peptone was used as an additional nitrogen source to the pre-optimized culture. From the present findings, it can be inferred that Trichophyton sp. (MBL 23) can be a prospective candidate for commercial lipase production. However, the stability analysis of the enzymes under stressful environmental parameters may be done before its final recommendation.
To Cite this article:
Download
