PJB-2000-3
CHLOROPLAST DNA VARIATION IN POLYGONATAE SENSU LATO (LILIACEAE)
ZABTA KHAN SHINWARI
Abstract
cp DNA variations were examined in 16 species of 7 genera of the tribe Polygonatae for 50 probe-enzyme combinations to study phylogenetic relationship among genera and species. The shared fragments were counted, index of similarity and distance matrix calculated. The study supports the idea against mono-phyly of Polygonatae sensu lato. The results suggest that there are three monophyletic groups within Polygonatae sensu lato. Group I with genera Maianthmum, Smilacina and Disporopsis; group11with Uvularia and Disporum and group III with genera Prosartes and Clintonia. The differences between each group (estimated F value x 100 were 5.88-7.8 for group I and 11, 4.8-6.6 for I and III and 3.4-5.61 for II and III. The distance within the genus Prosartes was 0.33-0.84, while it differed from Disporum in 3.4-5.52. The difference of Prosartes from Clintonia was 2.2, while between Disporum and Uvularia as 1.63-0.171. The RFLP studies turned out to be concordant to morphological data. The present findings support lumping of Smilacina in Maianthemum.
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