PJB-2015-77
THE EFFECT OF DIFFERENT GROWTH MEDIA, CARBON SOURCE AND PGRs ON DENDROBIUM BROGA GIANT ORCHID’S PROTOCORM-LIKE BODIES (PLBs) PROLIFERATION SUPPORTED WITH SEM AND TEM ANALYSIS
JASIM UDDAIN, PAVALLEKOODI GNASEKARAN, LATIFFAH ZAKARIA, CHEW BEE LYNN AND SREERAMANAN SUBRAMANIAM*
Abstract
Dendrobium Broga Giant (Dendrobium Bobby Messina × Dendrobium Superbiens) is a new orchid hybrid in Malaysia. The aim of this study was to investigate the effect of different growth media, carbon sources and PGRs on Dendrobium Broga Giant orchid’s protocorm-like bodies (PLBs) proliferation supported with SEM and TEM analysis. The PLBs were cultured on different strength of semi-solid and liquid MS medium and the proliferation rate was recorded based on the fresh weight basis. The maximum PLBs proliferation (12.31% } 0.57) was obtained in MS semi-solid medium. PLBs were cultured on MS semi-solid media supplemented with different sucrose concentrations (0, 10, 20, 30 & 40 g.L-1). The highest proliferation rate of PLBs (15.48% } 1.20) was recorded from MS supplemented with 20 g.L-1 sucrose. Different concentrations of BAP (0, 0.5, 1.0, 2.0 mg.L-1) and NAA (0, 0.5, 1.0, 2.0 mgL-1) were added to MS semi-solid medium. Combinations of 1.0 mg.L-1 BAP and 0.5 mg.L-1 NAA produced higher PLBs proliferation. Micromorphological studies by SEM and TEM displayed trichome, leaf primordial, stomata, various sizes of mitochondria, vacuoles, different shapes of chloroplast were found in PLBs which ameliorated the slow proliferation nature of plantlets.
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