PJB-2015-87
INFLUENCING FACTORS OF EMBRYO RESCUE IN SEEDLESS GRAPE
XIUWU GUO, WENLING CHEN, YINSHAN GUO*, ZHENDONG LIU, HONG LIN, JIAN TANG, KUN LI AND YUHUI ZHAO*
Abstract
In this study, we investigated the impact of inoculating stage, medium type and concentration of plant growth regulators on embryo rescue effectiveness by L25(5)6 orthogonal design using selfed ovules of ‘Venus Seedless’ as the testing material. The main results were as follows. The most important factor influencing ovule germination was inoculating stage. Ovule germinating rate gradually increased as inoculating being postponed. The highest germinating rate appeared when inoculation was done 55d after flowering. Other influencing factors were IBA concentration, exogenous amino acid, 6-BA concentration, GA3 concentration and medium type in descending order. The best embryo rescue result was based on Nitsch medium including 1.0 mg/L IBA, 0.1 mg/L GA3, 0.7 mg /L 6-BA and 2.0 mmol/L glutamine using ovules inoculated 55d after flowering. The highest germinating rate reached 41.25%, and a batch of seedlings was also obtained.
To Cite this article:
Download
