PJB-2015-97
ANALYSIS OF PAINT DEGRADATION BY FUNGAL AND BACTERIAL SPECIES
SHUMAILA ISHFAQ1, NAEEM ALI1, ISFAHAN TAUSEEF2, MUHAMMAD NASIR KHAN KHATTAK3, ZABTA KHAN SHINWARI4,5 AND MUHAMMAD ISHTIAQ ALI1*
Abstract
Paint is a liquor blend, used as a decorative or protective coating. Paints are the main source of volatile organic compounds (VOCs), very harmful for the environment and human beings. In the present study, fungal and bacterial growth on paint flakes sandwiched between the mineral salt medium agar layers were subjected to various analysis. Dry cell mass quantification was carried out by shake flask experiment with fungal inoculum. The maximum growth of 0.7g observed on 28th day. Further evidence of paint film biodegradation was confirmed by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) studies. The loss in intensity of the bands at a wavelength of 1115.7 cm-1 and 1065.67 cm-1 for ester linkages indicated degradation of the paints through the breaking of the ester group. A loss in intensity of bands at a wavelength of 3286.87 cm-1 (corresponding alcoholic peak) due to breakage of alcoholic linkages. Scanning electron micrographs clearly showed the adherence and fungal growth on paint flakes and the distorted / ruptured surface was also observed in three months treated paint samples. The current research study represents the significant trends of paint biodegradation by isolated microorganism.
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