PJB-2016-18
THE ACTIVITY AND GENE EXPRESSION LEVELS OF P5CS AND δ-OAT IN MEDICAGO SATIVA CV. PIANGUAN UNDER DROUGHT STRESS
HUI-SEN ZHU1, XIU-JU YU1, XIANG ZHAO1, WU-DE YANG2 AND KUAN-HU DONG1*
Abstract
Medicago sativa cv. Pianguan, an excellent local germplasm in Shanxi province, China, with the resistance to cold and drought is broadly planted. Many studies have showed that drought resistance of plants is positively correlated with the accumulation of proline, P5CS and δ-OAT play important roles in synthesis of proline. In this study, the proline content, activities and expression of P5CS and δ-OAT mRNA were detected in Medicago sativa cv. Pianguan under drought stress. The results showed that the proline content in Medicago sativa cv. Pianguan increased in the process of drought stress. The expression levels of P5CS and δ-OAT mRNA changed correspondingly at the different stress time point. In conclusion, P5CS and δ-OAT could participate in the drought resistance process by regulating proline content in Medicago sativa cv. Pianguan.
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