PJB-2001-20
CHARACTERIZATION OF MUSTARD [BRASSICA JUNCEA (L.) CZERN. & COSS.] GERMPLASM BY SDS-PAGE OF TOTAL SEED PROTEINS
M. A. RABBANI, A.A. QURESHI, M. AFZAL, R. ANWAR AND S. KOMATSU*
Abstract
A comparative study of total seed storage protein was carried out by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to characterize oilseed mustard [Brassica juncea (L.) Czern. & Coss.] germplasm from Pakistan. Oilseed collections from Pakistan as well as oilseed cultivars from diverse origin were not differentiated from each other nor were vegetable cultivars found to be distinct from one another on the basis of their seed protein patterns. Eight types of protein were recognized based on the banding patterns of 52 accessions. Relative inter-type relationships of protein patterns were estimated using Jaccard's similarity index and a dendrogram showing the hierarchical clustering was constructed by unweighted pair-group method with arithmetic averages (UPGMA). The clustering of eight protein types generally agreed with our previous classification and the limited data already available on inter-accession relationships in oilseed mustard based on morphological traits and RAPD analysis. The differences between the profiles of accessions 'PAK-85835', 'PAK-85839' and 'PAK-85910' supported the idea that they were separate species from B. juncea. The results showed that the technique of SDS-PAGE applied to seed proteins was not feasible to distinguish the closely related oilseed collections and cultivars from each other as they were characterized by the same banding pattern and formed a common gene-pool. However, seed proteins were useful to discriminate B. juncea and B. campestris. It was also possible to distinguish the oilseed mustard from the vegetable form. Future studies should involve a greater number of local accessions of oilseed mustard from other parts of Pakistan, and those of vegetable and condiment forms to further elucidate the situation.
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