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Comparision of in vivo and in vitro translation of the anemia phyllitidis poly (A)+ RNA during spermatogenesis
Abstract
Ribonucleic acid was extracted from 10 days old reproductive prothalli of the fern anemia phyllitidis . polyadenyiated RNA was isolated by using poly (U)- sepharose chromatography and its composition was studied by translating the poly (A)+ RNA in vitro in rabbit reticulocyte lysate. Electrophoretic analysis of the in vitro translation products and of proteins synthesized in vitro gave many common components , but there were also distinct quantitative and qualitative differences in the protein composition of the products of the two fractions . the in vitro synthesized proteins formed a heterogenous population , ranging in molecular weight from 10,000 to 72,000 , while those from in vitro ranged from 10,000 to over 120,000 daltons.
