Paper Details

PJB-1986-4

STUDIES ON IN VITRO CULTURE OF ARACHIS HYPOGAEA HYPOCOTYL EXPLANTS

IHSAN ILAHI AND SHAUKAT AMEEN
Abstract

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Arachis hypogaea hypocotyl explants exhibited callus formation when inoculated on Murashige & Skoog (MS) medium supplemented with both an auxin and cytokinin. Although best callus resulted in 0.5 mg/1 of 2, 4-D and 1.0 mg/1 of K but it did not support further callus proliferation. 2, 4-D @1.0 mg/1 and K @ 4.0 mg/1 supported callus growth. Browning of callus tissues in subcultures for long duration was overcome by the addition of coconut milk (CM) and sodium diethyl dithio carbo¬nate (SDDC).

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