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DETECTION OF ANTIFUNGAL COMPOUNDS FROM ANTAGONISTIC MICROORGANISMS INHIBITING THE GROWTH OF FUSARIUM OXYSPORUM F. SP. CICERIS
Abstract
Culture filtrate of the Cladosporium sp. (IC isolate) was found to inhibit 55% growth of Fusarium oxysporum f. sp. cicerts on Czapek dox medium (Oxoid). Another isolate (2C) reduced 24% growth and a strain of Bacillus subtitus inhibited 28% of the growth. The antifungal activity was found more in Czapek dox medium containing nitrite (NaNO, @ 2gl) as nitrogen source as compared to nitrate (NaNO, @ 3gV). The antifungal activity of the culture filtrate was best recovered into ethyl acetate at pH 3.00. Thin layer chromatography of the ethyl acetate and chloroform phases were done by using different solvent systems and the TLC plates were bionutographed by spraying test fungus which showed five inhibition zones produced by 1C isolate, representing five antifungal compounds and one by Trichoderma harzianum. Same inhibition zones werc observed when the TL plates were bioautographed against F. oxysporum f. sp. ciceris.
