PJB-2017-64
Response of tobacco polyphenol oxidase gene to wounding, abscisic acid (ABA) and methyl jasmonate (MeJ)
Ejaz Aziz, Wasim Akhtar, Muhammad Ilyas, Shazia Rehman, Hisashai Koiwa, Zabta Khan Shinwari and Tariq Mahmood
Abstract
Polyphenol oxidases (PPOs) are ubiquitous enzymes in plant kingdom and catalyze the oxidation of phenols to highly reactive quinones. PPO genes are induced by both biotic and abiotic stresses and implicated in several physiological processes including plant defense against pathogen and insects. Here, effect of wounding, abscisic acid (ABA) and methyl jasmonate (MeJ) applications on PPO induction in Nicotiana tabacum (NtPPO) was characterized by RT-PCR. In response to MeJ treatment at different concentrations, maximum expression of NtPPO genes was observed with six folds induction at 500 μM concentration, while target transcript level was relatively low (0.2-0.9 fold) with different concentrations of ABA. Upon wounding, NtPPO gene was strongly induced (up to 14 folds) after 36 hours treatment. Thus, wound and MeJ inducibility of NtPPO gene is a strong indicative of its role in plant defense mechanism against biotic and abiotic stresses
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