PJB-2017-92
Evaluation of D-genome synthetic hexaploid wheats and advanced derivatives for powdery mildew resistance
Khola Rafique, Chaudhary Abdul Rauf, Alvina Gul, Hadi Bux, Ahmad Ali, Rabia Asma Memon, Sumaira Farrakh and Abdul Mujeeb-Kazi
Abstract
The present study was undertaken to characterize 32 D-genome synthetic hexaploid wheats (SHWs) of an Elite-II sub-set and their 60 advanced derivatives. At seedling stage under controlled glasshouse conditions in Murree, the SHWs showed complete resistance/ immune to susceptible disease reactions viz., two (6.25%) were immune, eighteen (56.25%) were resistant, nine (28.12%) were intermediate, whereas three (9.37%) were susceptible. The BW/SH derivatives also demonstrated complete resistance/ immune to intermediate reactions viz., 51 (85%) were immune, 5 (8.33%) were resistant while 4 (6.66%) were intermediate. Adult plant resistance (APR) evaluated at the hill off-station summer site in Kaghan was 100% in SHWs (32/32) and 80% (48/60) in BW/SH derivatives. Several genotypes provided resistance at both plant stages viz., 20 (62.5%) SHWs and 45 (75%) BW/SH derivatives. All the entries were checked for the presence of Pm4b, Pm9, Pm16 and Pm30 resistance genes using linked SSR markers. The marker Xgwm382.2A flanked the gene Pm4b in one SH and 5 BW/SH derivatives. Pm9 gene was identified using two markers viz., Xgwm4.4A detected Pm9 in one SH and 27 BW/SH while Xgwm332.7A detected same gene in one SH and 13 BW/SH derivatives. Pm16 and Pm30 genes were present in one SH entry and in 2 BW/SH derivatives, detected by Xgwm159.5B. The findings revealed that 37.5% (12 entries) of the SHs and 5% (3) of the 60 BW/SH derivatives possessed APR. These observations add strength to exploit both intraspecific and interspecific strategies for allelic enrichment within wheat pre-breeding / breeding programs
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