PJB-2017-504
ASSESSMENT OF GENETIC DIVERSITY AMONG TURKISH SESAME (Sesamum indicum L.) GENOTYPES USING ISSR MARKERS
Cemal Kurt
Abstract
Information about genetic diversity is very crucial in plant breeding program because of crosses between parents with high inter-parental diversity may also help to develop varieties with broad genetic base. The genetic diversity and the relationships among 24 Turkish sesame genotypes were evaluated using 14 ISSR primers. The 14 ISSR primers amplified 68 bands of which 46 were polymorphic (68.2%). The polymorphism information contents (PIC) value, is the discriminatory power of the primers and used as a relative measure level of polymorphism, ranged from 0.06 0.70 with an average of 0.32. The 24 sesame genotypes were divided into 3 groups in a UPGMA based dendogram. Cluster A included 9 sesame population from different region of Turkey, while cluster B included only 5 sesame population from Adana province and two commercial cultivars (Muganli-57 and Baydar-2001). As to cluster C consist of 5 commercial cultivar which registered by same institute and three sesame population from east part of Turkey. This study gives some useful information about the genetic diversity of 24 sesame landraces. The variation harbored by these landraces could be used in sesame breeding program in Turkey as well as in the world. ISSR primers grouped some landraces based on their geographical provenances and while cluster others showing that ISSR could be useful in geographical clustering of sesame landraces.
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