PJB-2017-506
GENETIC VARIABILITY STUDY OF DIVERSE GUAR (CYAMOPSIS TETRAGONOLOBA L.) GERMPLASM AS REVEALED BY SDS-PAGE METHOD
Sohail Ahmad Jan
Abstract
Biochemical characterization of plant species through SDS-PAGE method play important role in seed storage protein based variations. Limited reports are available about the protein profiling of diverse Guar germplasm. In present study we have characterized the seed storage protein profiles of diverse accessions of Guar. The twenty four Guar germplasm were characterized with standard SDS-PAGE protocol with minor modifications. These genotypes were also analyzed with novel 2D and 3D method to visualize diverged genotypes from close angle. A huge level of variability was observed in protein profile of all tested genotypes. The banding sizes varied from 10-180kDa. Maximum (100%) polymorphic banding patterns were observed among genotypes. The genetic similarity values ranged from 20 to 100% between genotypes. The genotypes 28952 and 31682 showed highest genetic similarity value (100%). While the lowest similarity value (20%) was recorded between 31731 and 31764. All these genotypes were classified into seven different groups and the last three were highly diverged as compared to others. The 2D and 3D protein structure models identified three unique accessions like 31764, 31731 and 31761 from very close angle. The resulted unique/novel genotypes will be useful for further improvement of this important plant species.
To Cite this article:
Download
