PJB-2017-523
INTRA-SPECIE PROFILING OF CLEOME VISCOSA L. GROWING IN DISTRICT SWAT KHYBER PAKHTUNKHWA PAKISTAN
Noor Muhammad1
Abstract
The present study was carried out to investigate intra specific genetic variation in twenty eightgenotypes C. viscosa L.growing in Swat, KP, Pakistan, on the basis of morphological characterization and seed storage protein. It was found that genotypes showed the utmost allelic variation for leaf upper and lower surface with Emarald green (75%), and yellow green (75%) respectively other leaves lower and upper surfaces were with (25%) green and yellow green (26%) respectively. Majority of C. viscosa L. genotypes were with (50%) yellow colour flowers while other was with (28.57%) White yellow colour and (21.42%) Dull yellow. Most of the seeds were with black (46.42%). The landraces present seeds with others colors, reddish brown (14.28%), brown (39.28%) and mostly with ovate (78.57%) while other with oblong (17.85%). Mostly the seeds have coloured hilum. The colour of hilum can range from yellow (39.28%), white yellow (17.85%), and white (42.85%). Regarding the testa texture, the seeds can be classified in two groups: rough (67.85%) or smooth (32.14%).The data showed that there is considerable morphological variation among the genotypes of C. viscosa. The protein profiling was carried out on 12% slab gel electrophoresis; seven reproducible bands with molecular weight ranges from 180KDa to 10KDa were detected in C. viscosa L. the locus contribution toward genetic disagreement (LCTGD) of C. viscosa was 57.14%.Notably, L-3, L-4 L-5, was monomorphic in C. viscosa and was treated as specie specific. L-1, L-2, L-7 were polymorphic. These bands showed 78.57, 3.57, 14.28 and78.57 respectively. In this study intra-specific variation was limited and it was observed that SDS-PAGE alone did not exhibit high level of intra-specific variation; therefore, diverse germplasm based on SDS-PAGE is suggested to be acquired from various sources, preferably from center of diversity to build a broad based gene pool with maximum variability.
Key word: Cleome viscose L. Genetic diversity, Morphology, SDS-PAGE
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