PJB-2017-526
IN SILICO PHYLOGENETIC ANALYSIS OF FUNGAL LIPASE GENES AND HARNESSING THE INHERENT POTENTIAL OF ASPERGILLUS NIGER IBP2013 FOR EXTRACELLULAR TRIGLYCEROL ACYL-HYDROLASE PRODUCTION UNDER SOLID STATE FERMENTATION
DUR-E-NAYYAB
Abstract
Mesophilic fungi have evolved inherently thermostable/thermophilic enzyme production potentials over the years. To explore this potential, fungal colonies were isolated from rotten food items and screened based on their extracellular triglycerol acyl-hydrolase (lipase) potential over lipid-rich agro-wastes. Hyper lipase producing fungus was identified by comparing with standard fungal morpho-graphs as Aspergillus niger IBP2013. For the first time, coconut meal was found a suitable solid culture media for the fungus to produce higher extracellular lipase enzyme (lipase activity (la), 23.7�0.25 U/mL min-1; lipase specific activity (lsa), 97.4 U/mg) under solid state fermentation. This inherent potential of the fungus was enhanced up to 41.1�0.4 U/mL min-1 (la) and 169.5 U/mg (lsa) by eco-cultural optimization like substrate (coconut meal) and adequate concentrations (10 g), inoculum level (1 mL), incubation period (72 h), temperature (40� C) and pH (7). Moreover, the concentrations of organic (peptone, 1% w/v) and inorganic nitrogen (ammonium acetate, 1% w/v) and carbon (lactose, 0.8% w/v) supplements were found essential for optimal lipase production. Three known fungal genes (Lip1, Lip2 and Lip3) were utilized for in silico phylogenetic analysis. Hence, A. niger IBP2013 with optimized cultural conditions could be potential candidate for lipase production at commercial scale.
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