PJB-2017-577
IDENTIFICATION OF STRIPE RUST RESISTANT GENES IN D-GENOME BASED SYNTHETIC HEXAPLOID WHEAT
Rumana keyani
Abstract
Stripe rust (Puccinia striiformis) is significant biotic stress constraint that influences bread wheat productivity globally. Use of genetic diversity in wild relative and domestic wheat, D genome diploid accessions of Aegilops tauschii are targeted via their presence in synthetic wheats (T.turgidum / Ae. tauschii, 2n=6x=42, AABBDD) which is advanced derivatives from the synthetic hexaploid (SH) x bread wheat (BW). The objectives of this study were to phenotype 53 synthetic hexaploid of wheat and identification of 3 major stripe rust resistant genes,(Yr5, Yr15, Yr18) in order to identify potential parental lines for breeding programs. For phenotyping, traits such as plant height, cycle and grains characteristics were assessed. The result of the study showed that all of the lines possess very strong agronomic characters. grain weight per spike was and 26.1�12.2 g. Spike length ranged from 8cm to 15cm, plant height ranged from 81cm to 125cm. Marker assisted selection showed that Yr5 was identified in 1, Yr15 was identified in 4 and Yr18 was identified in 18 of these synthetic hexaploid of wheat. It is concluded that these SH carried variation in both agronomic traits and Yr genes therefore, strategies for deploying these resistance genes to prolong effective disease resistance are suggested to control wheat stripe rust disease in Pakistan
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