PJB-2017-585
An insight into in vivo and in vitro pharmacological activities of Pinus wallichiana; a new era of herbal medicine
ibrar khan
Abstract
The aim of the present study was to screen the test samples (Cr. Met. Ext and different fractions of stem, leaves and resin) of Pinus wallichiana for possible in vitro and in vivo pharmacological activities. The aqueous fraction of leaves presented good antibacterial activity against VRSA (63%), EtOAc fraction of resin against A. baumannii (60.8%) and Cr. Met. Ext of the stem against P. vulgaris (62%), S. aureus (61.5%) and E. coli (60.3%). The test samples showed low to no activity against test fungal pathogens. The phytotoxic activity of test samples was moderate to low at 1000 ?g/ml. The results of haemagglutination indicated lack of phytoagglutinins in P. wallichiana. The antioxidant activity was good to moderate at 500 ?g/ml and the activity was concentration dependent. Good anti-termite activity was shown by the test samples against H. Indicola while significant activity (100%) against the selected test insects. The Cr. Met. Exts were found safe at the tested dose and no lethality was observed in acute toxicity assay. The Cr. Met. Ext of P. wallichiana stem, resin and leaves presented significant decrease in the number of writhes after intervals of 30, 60 and 90 minutes in a dose dependent manner. The percent GIT motility observed in test group treated with different concentration of P. wallichiana stem, leaves and resin was 46.35,49.93 and 42.72% at 100mg/kg, respectively and same decreased was observed at 200 and 300mg/kg. The Cr. Met. Exts were observed to have significant anti-pyretic effect after 1, 2 and 3 hours. Key words: antibacterial, antifungal, antioxidant, acute toxicity, GIT motility, anti-pyretic
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