PJB-2017-630
METABOLIC MECHANISM OF ASCORBIC ACID IN ACTINIDIA CHINENSIS VAR. CHINENSIS
JI-YU ZHANG
Abstract
Ascorbic acid (AsA) concentration was determined in Actinidia chinensis var chinensis ‘Jinyang’ and ‘Hort16A’ during fruit development. AsA concentration was higher at early stage of fruit development, and then declined with fruit development. The expressions of L-Galactose pathway genes were detected and the results showed GMP1, GME1 and GDH1 genes transcripts were declined with fruit development. Statistically significant correlations analysis results showed that there were significant positive correlation for GMP1, GME1 and GDH1 expressions with AsA concentration, and the expression level relations between each pair of GMP1, GME1, GDH1 are significant positive correlation in these two species, suggesting that GMP1, GME1 and GDH1 in L-Galactose pathway play important roles in AsA accumulation in A. chinensis var chinensis. The trend of AO1 gene transcript was corresponding with the AsA concentration, and there was a significant positive correlation between AO1 expression and AsA concentration in these two species. The expression of four MDHAR genes and one DHAR gene were different between two A. chinensis var chinensis varieties, predicting that DHAR or MDHAR genes might not key genes for AsA biosynthesis in A. chinensis var chinensis.
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