PJB-2017-635
GC-MS RESULTS FOR n-HEXANE EXTRACT OF THE AREIAL PLANTS OF ASTRAGALUS ALOPEIAS PLANT AND ANTIMICROBIAL ACTIVITY
ali dehjurian
Abstract
Astragalus is considered as the greatest genus of vascular plants. As of now, 2500 – 3000 species and 250 sections of Astragalus are discovered. This study was conducted to determine chemical compounds present in hexanoic extracts of the flower and leaf of Astragalus alopecia, for the first time in Iran. Gas chromatography and mass spectroscopy were used to analyze the compounds. According to the results, the extracts were mostly composed of alkanes, alkaloids and esters. 35 compounds were detected in flower extract. The main compounds are Decane (2.8%), Dodecane (5.2%), trans,trans-1,10-Dimethylspiro (3.6%), Hexadecane(3.5%) . The leaf extract contained 24 compounds, of which the three main compounds were Octadecane(4.5%), Hexadecane 94.3%) and 9,12-Octadecadienoic acid 93.3%). Subsequently, antimicrobial activity of methanolic and hexanoic extracts of the Astragalus were investigated on several bacteria. The extracts were tested in disc diffusion assays against Staphylococcus epidermidis, Escherichia coli and Klebsiella pneumonia. The results of antibacterial activity analyses revealed that, all of the tested extracts showed good inhibitory activity against all of the tested pathogens. The activity of the tested pathogens. The activity of the extract was further compared to those of standard antibiotics.
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