PJB-2017-643
EXPRESSION PROFILING OF DEFENSE RELATED GENES AFTER SYSTEMIC INOCULATION OF Arabidopsis thaliana BY Macrophomina phaseolina
Saima Khalid
Abstract
Infection caused by a necrotrophic fungal pathogen Macrophomina phaseolina leads to certain changes in its growth, development, and metabolism in a model plant Arabidopsis thaliana. Defense responses produced include the induction of expression of different pathogenesis related (PR) proteins. Results obtained indicated alterations in some growth (shoot length, root length) and physiological (photosynthetic pigments, relative water content (RWC), sugar content and proline content) parameters of A. thaliana was recorded. M. phaseolina infection reduced the RWC and chlorophyll content but increased sugar and proline content in leaves. Analysis of gene expression showed that among (PR) proteins, the expression of Mitogen-Activated Protein Kinases and thaumatin proteins were highly increased while Chitinase and beta-1,3-glucanase showed little up regulation. These results proved significant differences in the gene expression profiles between the control and the infected plants. It is, therefore, suggested that these genes along with sugar and proline contents enhance the development of resistance against stress A. thaliana.
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