PJB-2017-666
Screening of EMS-induced NaCl-tolerant mutants in Cucurbita moschata D.
Xuejin Chen
Abstract
Salinity is one of the major constraints impairing plant growth and ultimately limits crop productivity. Breeding salt-tolerant crop cultivars is thought to be an efficient strategy to make use of the saline land. Pumpkin (Cucurbita moschata D.) is economically important and widely cultivated vegetable worldwide. EMS (ethyl methanesulfonate), as a mutagenic agent, has been widely used to increase genetic variability in crop breeding. In this study, we designed a screen of NaCl-tolerant mutants induced by EMS in Cucurbita moschata D. Two NaCl-tolerant mutants were screened under NaCl stress and further identified using physiological and biochemical parameters. As the result of genetic analysis, we deduced the NaCl-tolerant mutant phenotype was controlled by QTLs. This research provides the protocol of mutant screening and new sources for salt-tolerant cultivar breeding in pumpkin. The mutants can also be used to better understand the salt-tolerance mechanism in plants.
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