PJB-2017-668
Detection of genetically modified maize in Jordan
ABEER ABURUMMAN
Abstract
The present study was carried out for the detection of genetically modified maize (GMM) in seeds of eleven imported maize hybrids grown in Jordan. Promoter 35S and T-nos terminator were used for the general screening of transgenic materials. The specific events were detected by conventional PCR for screening of Bt 11, Mon 810 and Bt 176 events. Seeds of eleven maize hybrids samples showed positive response to the presence of the 35S promoter, nine out of eleven showed positive response for T-nos terminator. Bt 11 event was the most commonly used in GMM seeds, where seven out of eleven samples showed positive results. Two out of eleven hybrids showed presence of Bt 176 event, however, Mon 810 was not detected in any of the tested hybrids. The Bt 11 event was studied in imported GMM seeds in Jordan for the first time, reinforcing the need for mandatory labeling system and a valid simple qualitative method in routine analysis of GMCs.
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