PJB-2017-670
INFLUENCE OF ZINC-SOLUBILIZING BACTERIA AND ORGANIC ACIDS ON ZINC UPTAKE AND GROWTH OF RICE USING ZINC SULFATE FERTILIZER
nur maizatul idayu othman
Abstract
An Investigation on the influence of Zinc-solubilizing bacteria (ZSB) inoculation, Zn fertilizer and organic acids on growth of rice was done at Microbiology lab, Universiti Putra Malaysia. Commercial oxalic acid (BDH), malic acid (SIGMA), citric acid (SIGMA), succinic acid (SIGMA) were used to solubilise Zn oxide, Zn carbonate and Zn sulfate at four different rates (0, 0.1, 1.0 and 10 mM). Selected organic acid and its rate then combined with ZSB and Zn Sulfate using sand culture experiment for rice plant treatment. Citric acid showed significantly highest in Zn sulfate solubilisation activity at 0.421 mg L-1. Similar observation found for malic acid and oxalic acid in Zn sulfate at 0.331 mg L-1 and 0.249 mg L-1 respectively. Then, selected organic acids were malic acid and citric acid for plant treatment with Zn-solubilizing bacteria (ZSB) and Zn sulfate. Highest plant biomass was found in inoculated plants treated with 0.1 mM malic acid in the presence of Zn sulfate at 135.67 mg. 3 plants -1 .The same treatment also observed for highest plant height at 29.57 cm. With addition of malic acid, the population of rhizosphere, endosphere and non-rhizosphere were varied, but higher than control treatment Application of ZSB inoculation, with Zn sulfate at 0.2 mg L-1 worked best with malic acid at 0.1 mM concentration due to high increase of rice plant growth parameters, Zn uptake and Zn concentration. Bacterial populations were also varied due to different organic acids and its rate.
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