PJB-2017-685
Ectopic expression of aldehyde dehydrogenase 3I1 gene confers abiotic stress tolerance in plants
HASNAIN RAZA
Abstract
Plant growth and productivity is restricted by a multitude of abiotic stresses. These stresses affect physiological and metabolic pathways leading to the production of many harmful substances like reactive oxygen species, lipid peroxides and aldehydes just to name but a few. The study was envisaged to investigate whether the overexpression of Arabidopsis ALDH31I genes augment the ability of plants to tolerate stress. For this purpose, transgenic tobacco plants overexpressing the ALDH3I1 gene were generated and evaluated under various stresses. Transgenic plants flaunted elevated ALDH3I1 transcripts expression signals than wild type plants. The constitutive ectopic expression of the ALDH3I1 conferred increased tolerance to salt, drought and oxidative stresses in transgenic plants along with improved plant growth. Analyses of chlorophyll levels and photosynthesis rates revealed the positive contribution of ALDH3I1 overexpression in stress tolerance. The transgenic plants produced less ROS and MDA molecules, and accumulated higher amounts of proline than wild-type counterparts under drought stress. Thus, ALDH3I1 overexpression improves stress tolerance in plants. This study has future implications in producing transgenic cereal and horticultural plants tolerant against abiotic stresses.
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