PJB-2017-696
MOLECULAR AND CHEMICAL CHARACTERIZATION OF MUTANT GENOTYPES OF SAFFRON GROWN IN SAUDI ARABIA
MAHMOUD A. SHARAF-ELDIN
Abstract
Crocus sativus is an important food and medicinal plant cultivated in Asia and Europe. Morphological and biochemical parameters such as tepal, stigma, biomass, chlorophyll content were mainly affected by environmental conditions. A RAPD-PCR approach was studied to analyse the degree of polymorphism between Crocus sativus genotypes grown in Saudi climate. In this research study, DNA fingerprints of mutated and non mutated stigmas of C. sativus genotypes (CM-Kashmirian, T0, T1-2B, T4-2A) were developed and a total of 10 decamer primers were used for PCR amplification (RAPD-PCR) analysis. Only number three arbitrary primers showed polymorphism (33.3%-88.2%) using the whole genomic DNA extracted from these genotypes (CM-Kashmirian, T0, T1-2B, T4-2A). Jaccard’s similarity index (JSI) ranged between 1.0 and 0.88. Based on Unweighted Pair Group Method with Arithmetic Mean (UPGMA) similarity and dendogram matrix, genotypes (T1-2B, T4-2A) were closely related to each other and the strain CM-Kashmirian, T0-Crocus sativus genotypes showed more divergence.
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