PJB-2017-699
THE AMELIORATING EFFECTS OF POLYAMINE SUPPLEMENT ON PHYSIOLOGICAL AND BIOCHEMICAL PARAMETERS OF STEVIA REBAUDIANA BERTOLI UNDER COLD STRESS
Kamran Moradi
Abstract
Stevia rebaudiana Bertoni. is a perennial medicinal plant indigenous to tropical regions of south America. Present study evaluate some physiological changes of the plant under cold and cold+ polyamine conditions. The results showed photosynthetic pigments and photochemical efficiency of PSII were significantly reduced in cold treatmen courses than control, but in all of polyamine treated goups a considerable increase in these parameters was seen. Leaf compatible solutes such as proline and glycine betaine that were not shown significantly increasing at cold treatment groups, were elevated in the 48 h cold-polyamin treated group. The similar results were depicted for free amino acid and total protein contents of the treated plants. It was also revealed that specific activity of some antioxidant enzymes such as catalase and ascorbate peroxidase were increase in cold treated or cold + polyamine treated groups than related controls. It caused a significant decrease in H2O2 and malondialdehyde contents of the treated plants. The results indicated a increasing in saturated long chain fatty acids after 96 h of cold at 4◦C. SDS- PAGE analyses of the plant leaves proteins displayed that in cold and cold-polyamin treated groups of Stevia some electrophoretic protein bands density were increased than control
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