PJB-2017-710
DNA damage protection studies by ultraviolet/ microwave radiations and H2O2 treatment using Onosma hispidum (ratanjot)
Khalida Perveen
Abstract
In this study the methanolic extract of Onosma hispidum (O. hispidum) was investigated for its DNA protective activity against hydrogen peroxide, ultraviolet and microwave radiations damage by using gel electrophoresis method. The plasmid DNA was exposed to ultraviolet and microwave radiations in the presence and absence of O. hispidum extract. Exposure to ultraviolet, microwave radiation or hydrogen peroxide treatment led to induction of DNA strand breaks, resulting in the relaxation of plasmid DNA from super coiled form to open circular form. Gel electrophoresis results revealed that the plant extract at 1μg concentration was protective against hydrogen peroxide, ultraviolet and microwave radiations induced DNA fragmentation. The extract was analyzed for total phenolic and flavonoid contents and antioxidant activity using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. Antioxidant activity of extract was expressed as percentage of DPPH radical inhibition and values in percentage ranged from 37.97 to 83.45%. The anti-biofilm activity of O. hispidum extract was also tested by microtiter-plate method. Best DNA protective efficacy of O. hispidum was observed for Bacillus subtilus biofilm at concentration of 5 μg/ml. These observations emphasized that the methanolic extract of O. hispidum possess high antioxidant and DNA damage inhibition potential Therefore the investigated plant can be used for therapeutic use against DNA stress as natural shield.
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