PJB-2017-711
The effect of temperature and different germination medias on pollen germination and tube growth of Betula utilis D.DON an endangered plant of Kashmir Himalaya
Mohammad Saleem Wani
Abstract
Betula utilis is an important tree species of Himalayan region which is placed in endangered category. The present study was carried out to determine the effect of four different temperatures (5, 15, 25 -30 and 35°C) on the pollen germination of B. utilis. Different culture medias were also tested in an attempt to improve the knowledge of the most suitable germination media for studying in vitr Koo pollen germination capacity from Gulmarg area of Kashmir Himalaya. The research was conducted with different concentrations of sucrose (1%, 3%, 5%,10% and 15%), H3BO3 (25 ppm, 50 ppm, 100 ppm and 200 ppm), Ca(NO3)2 (50 ppm, 100 ppm, 200 ppm, 300 ppm and 400 ppm), MgSO4 (50 ppm, 100 ppm, 200 ppm and 300 ppm)and KNO3 (50 ppm,100 ppm and 200 ppm). At 10°C, the average pollen germination was 12.9%. This increased to 32.3% between 15 and 25°C, and subsequently decreased to 23.2% at 30°C. Pollen germination was maximized (98±6.1%) when the germination medium contained 10% sucrose solution supplemented with 100 ppm boric acid, 300 ppm Ca(NO3)2, 200 ppm MgSO4 and 100ppm KNO3, after 6 hrs of incubation. In addition our results revealed that pollen grains collected immediately after anther dehiscence showed the best germination ability but gradually decreases with time. Pollens collected at different times showed that viability was highest at the time of anthesis.
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