PJB-2017-715
PHYTOCHEMICAL SCREENING, ACETYLCHOLINESTERASE INHIBITORY, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF ANTHYLLIS SERICEA EXTRACTS FROM SOUTHERN OF TUNISIA
AYET SELMI 1,2,*
Abstract
This study was aimed to reveal the anti-acetyl cholinesterase activity, Oxygen Radical Antioxidant Capacity (ORAC), antioxidant activity by Radical Scavenging Capacity (ABTS) of Anthyllis sericea leaf. Water, ethyl acetate and ethanol extracts were screened for phytochemical constituents of Anthyllis leaves. Antimicrobial activity of the extracts was assessed by the diffusion method against 5 bacteria and 2 fungi. Alkaloids, phenolic compounds, flavonoids, tannins, saponins, carbohydrates and essential oils were screened. The ethanol extract had highest quantities of phenols and flavonoid with 422.36 mg gallic acid equivalents/g and 129.67 mg quercetin equivalents/g of dry weight respectively. This tested solvent exhibited an antioxidant activity by ABTS scavenging activity test (IC50= 0.04 mg/mL). An effective anti-acetyl cholinesterase activity with IC50= 0.032 mg/mL and ORAC values were observed with the ethanol fraction. The extracts had moderate antimicrobial activity against reference fungi and bacteria with inhibition zone ranging from 20-11 mm and MIC values between 1024-64 μg/mL respectively. These obtained leaves proprieties of Anthyllis sericea confirm that could be a powerful source of natural antioxidants.
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