PJB-2017-718
EVALUATION OF GENETIC VARIABILITY IN THE REGENERATED POPULATION OF SUGARCANE
Shahla Karim Baloch
Abstract
Direct regeneration is an important method for the large scale manufacturing of infection free sugarcane planting material of the presented varieties and can likewise help in quick presentation and dispersion of new varieties through fast multiplication. Rapid and efficient protocol was used in sugarcane through in vitro culture for direct regeneration. The MS average incremented among various concentration of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA), kinetin (Kn) and 2, 4-Dichlorophenoxyacetic acid (2, 4-D) were used for through regeneration in sugarcane. The results indicated that maximum number of shoots with shoot length were observed in NIA-2004 variety on MS average incremented with 6.00 mg l-1 IAA + 1.00 mg l-1 kinetin and 0.5 mg l-1 2, 4-D, minimum number of shoots plus shoot length were recorded in NIA-98 with concentration of MS + sugar 40 g l-1 under control. Development of chlorophyll mutant conforms that direct regeneration cannot uphold hereditary loyalty but could be considered as a good sources exploring existing aneuploidy. The maximum number of roots was practical in NIA-2011 under the concentration of MS + 1.00 mg l-1 IBA + 20 g sugar while minimum number of roots was practical on MS + 1.00 mg l-1 IBA + 10 g sugar in NIA-98. The maximum root length was obtained in NIA-2011 under the concentration of MS + 1.00 mg l-1 + 20 g l-1 sugar and minimum root length was achieved in NIA-98 under the concentration of MS + 1.00 mg l-1 + 10 g l-1. It was concluded that the best shoot induction was observed under the concentration of MS average including 6.00 mg l-1 IAA + 1.00 mg l-1 kinetin + 0.5 mg l-1 2, 4-D + sugar 40 g and MS + 1.00 mg l-1 IBA + 20 g sugar proved best concentration for root induction for direct regeneration in sugarcane
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