PJB-2017-720
GENOME SIZE AND MORPHOLOGICAL VARIATION IN Brachypodium distachyon L. ALONG ALTITUDINAL LEVELS
Signem Oney-Birol
Abstract
We determined genome size and morphological variations under different altitudes in Brachypodium distachyon L. (Poaceae). Flow cytometric analysis was performed with 547 individuals representing 89 accessions of B. distachyon from different altitudinal habitats (from 0 to 1219 meters) of Turkey. 2C nuclear DNA content (± SD) of B. distachyon was estimated to be 0.736 ± 0.02 pg. In cytological analysis, all the individuals from every accession were found to have diploid chromosome numbers (2n = 10). To determine the association with genome size (GS), morphologic traits and altitude obtained data performed using statistical procedures such as ANOVA (P< 0.005), pearson correlations (P< 0.005), principal component analysis, factor analysis, discriminant analysis in Minitab 17 and SPSS 22 versions. The correlation analysis shows that there is no correlation between genome size and altitude. To see the changes of the morphologic variation, 5 morphological features such as plant height (13.26± 5.39 cm), plant stature (mostly erect), seed height (7.34± 0.89 mm) and awn lenght (10.57± 2.24 mm), and 1000- seed weight (4.21± 1.00 mg) was used. We also have not found any correlation with changing altitudinal gradients and morphology. However, when we grouped the altitudes between 0-600m and 601-1219m we have found a positive correlation between genome size, seed height, awn lenght, 1000-seed weight and altitude. A negative correlation was only found in plant height along increasing altitudinal levels.
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