PJB-2017-758
GENETIC SEPARATION OF CHALKINESS BY HYBRID RICE OF HUANGHUAZHAN AND CS197
LU GAN
Abstract
Percentage of grains with chalkiness (PGWC) controlled by multi-genes is one of the main appearance quality of rice. The selection among the offspring using genetic separation directly influences quality improvements of hybrid rice. The present study focused on the segregation of PGWC, using Huanghuazhan as the female parent and CS197 as the male parent to construct hybrid rice F2 population. Molecular markers were used for genotype analysis among the extremely low and high PGWC individuals from the F2 population. The results revealed that the genotypes of 10 extremely low PGWC individual plants were 80.00% and 3.33% identical to that of Huanghuazhou and CS197, respectively. The heterozygotes accounted for 16.67%. Genotype and phenotype of the most 10 individual plants were the same as Huanghuazhan. On the contrary, the genotypes of 10 extremely high PGWC individual plants were 37.78% and 32.22% identical to that of Huanghuazhou and CS197, respectively. The heterozygotes accounted for 30.00%. Genotype and phenotype of the most of 10 individual plants were the same as CS197. The extremely low and high PGWC individuals had high coincidence with the corresponding F2:3 family lines in phenotype and genotype. Scanning electron microscopy (SEM) images showed that the shape and spacing of starch granules in the extreme low and high PGWC individuals were similar to that of Huanghuazhan and CS197, respectively. Correlation analysis showed that the F2 strains showed a normal distribution trending to Huanghuazhan. The broad sense heritability was 26.31% and the narrow heritability sense was 17.21%. The results of cluster analysis showed that the range of PGWC variation of the group III based on phenotype and the group II based on genotype varied from 5.66% to 50.00% with an average of 25.46% and from 14.34% to 60.67% with an average of 32.33%, respectively. The group III and II shared 28 identical strains. This is the first report on the genetic separation of PGWC in Huanghuazhan.
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