PJB-2017-767
CONSTRUCTION OF MICROALGAL BASED CARBON PASTE ELECTRODE BIOSENSOR FOR THE DETECTION OF Hg(II) AT PPB LEVEL IN REAL WASTEWATER
Asma Saeed
Abstract
Mercury is one of the persistent environmental pollutants and its detection has gained massive attention these days. The study exploited the ability of unicellular red microalga, Porphyridium cruentum based carbon paste electrode (PCBCPE) biosensor to detect Hg(II) in wastewater. Hg(II) is initially deposit as Hg0 on the surface of electrode by applying deposition potential of -0.5V which afterwards stripped off using anodic scan between -0.5 to +0.5V. Experimental parameters like biomass to carbon ratio, deposition potential, accumulation time, pH of accumulating medium were optimized. Best response of PCBCPE biosensor for Hg(II) was obtained using 10% biomass at pH 7. The minimum detection limit for Hg(II) was found to be 0.95 µg/L. The efficiency of PCBCPE biosensor was studied in the presence of several interfering ions. The PCBCPE biosensor successfully dectected/measured Hg(II) from real industrial wastewater collected from local chlor alkali plant located in Lahore, Pakistan.
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