PJB-2017-771
MOLECULAR IDENTIFICATION OF NOTEWORTHY LIGNICOLOUS FUNGUS, NEOLENTINUS LEPEDIUS: A NEW GENUS FOR PAKISTAN USING PHENOTYPICAL AND PHYLOGENETIC APPROACHES
Abdul Razaq
Abstract
Neolentinus lepedius is a lignicolous/saprophytic macrofungus found on the dead logs of pine vegetation of Himalayan forests, not previously reported from Pakistan. The purpose of this work is to report the occurrence of new record European genus and its species from pine forests of Pakistan using morphological and molecular characterization. For species recognition, the phenotypic features like size, shape, colour of basidiocarp and scales on pileus, length, width and texture of stipe and spores have been compared with available literature. Internal Transcribed Spacers (ITS) region of ribosomal DNA of all local collections has been amplified by using Polymerase Chain Reaction (PCR) technique with ITS1f and ITS4 primer pair. Molecularly, the species sequences were confirmed by using BLAST analysis and construction of Phylogenetic tree. All the data analysed was found consistent with morpho-anatomical and molecular characters of N. lepideus. Morphological description, photographs, line drawings and phylogenetic tree of Pakistani collections have also been provided.
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