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PJB-2003-71

TOWARDS A SEEDLESS CULTIVAR OF KINNOW MANDARIN VI. PRODUCTION OF SEEDLESS PLANTS BY SPROUT/ SHOOT APEX/EMBRYO GRAFTS

NAFEES ALTAF AND M. MOHSIN IQBAL

Abstract

The work reported relates to grafting of sprout/shoot apex/nucellar embryo of Kinnow tissue having seedless trait on rough lemon seedlings. Normal healthy and vigorous embryos with balanced germination were top grafted on 2 - 18 months old soil established healthy seedlings with 76% survival. Rough lemon seedlings with dense foliage, healthy, green, vigorous stem from fleshy vegetative shoots with sprouting leaves, balanced (top) shoot - root growth were found ideal for grafting shoot apical meristem (SAM) with 6 inches top stem with leaves having axillary meristem showed best graft survival (85%) and good source of micro plant propagation.

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