PJB-2017-778
Development and Characterization of 28 Polymorphic EST-SSR Markers for Rhododendron hybridum Hort (Ericaceae)
chenminghui
Abstract
Rhododendron hybridum Hort. (Ericaceae) is an important ornamental species with continuous flowering feature. Highly valuable polymorphic EST-SSR markers were developed for investigating the geospatial and ecological diversity, which was useful for designing appropriate conservation and breeding measures. A total of 144 microsatellite primers were designed on the basis of the transcriptome data of R. hybridum. Of them, 28 EST-SSRs showed high polymorphism by genotyping 60 individuals from five representative populations. The number of alleles per locus varied from two to thirteen, the observed heterozygosity ranged from 0.029 to 1.000, and the expected heterozygosity ranged from 0.028 to 1.000. A total of 21 primers amplified in R. simsii, and 18 primers amplified in R. indicum. The EST-SSR markers described here will be useful in studies on genetic variation, population genetic structure, and gene flow of this species. These markers are a valuable resource for designing appropriate breeding measures for the related Rhododendron species.
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