PJB-2017-795
IN VITRO PROPAGATION OF THREATENED BRASTAGI CITRUS VARIETY BRASTEPU (Citrus nobilis BRASTEPU) FREE CVPD THROUGH SHOOT TIPS SUBCULTURE
ISNAINI NURWAHYUNI
Abstract
Abstract In vitro propagation of threatened Brastagi citrus variety Brastepu (Citrus nobilis Brastepu) through shoot tip subculture was carried out as steps to obtain good quality citrus seedling free from CVPD. Propagation of citrus plants via cutting bud technique was firstly conducted to resque rare plant as well as to provide adequate shoot tip explantsfor in vitro propagation. Shoot tip explants of Citrus nobilis Brastepu were cultured in MS basal media supplemented with combinations concentrations (0-2 mg/L) of 2,4 dichlorophenoxyacetic acid (2,4-D) and (0-3 mg/L) of benzyl-amino purin (BAP) followed by subculture techniques. The results showed that subculture frequency and combination variations of growth stimulator become key role to success of citrus growth development. The best culture was obtained in D1B2 treatment of the second subculture, that was with supplementation of 0.5 mg/L 2,4-D and 1.0 mg/L BAP, resulted in the average weigh of callus (2.30 g), number of embryosomatic (22.30), and the number of shoot (8.60) respectively. Subculture technique was chosen to provide nutrients for long periode of incubation time. Subculture technique has improved the quality of citrus culture and be able to produced good quality citrus plantlets. Citrus culture has developed to become normal plant in which the stem, leaves, and roots are generated. The plantlets of Citrus nobilis Brastepu were all free from CVPD. The method was potential to be usedas steps for mass production of Citrus nobilis Brastepu seedling to conserve threatened local Brastagi citrus.
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