PJB-2017-804
Effects of exogenous abscisic acid on expression of cold-regulated genes in winter wheat under low-temperature stress
Lijie Liu
Abstract
This study aimed to explore the effects of exogenous abscisic acid (ABA) on expression of cold-regulated genes in winter wheats under low-temperature (LT). Dongnongdongmai 1 (DNDM1) and Jimai22 (JM22) were treated with ABA at 4 °C for 0, 0.5, 2, 8, 24, 48, 72, 96, 120, 144 and 168h, and at 4, 0, -10 and -25°C. RNA was extracted by Trizol and the expression levels of genes were assessed by Quantitative real-time PCR. Moreover, the levels of ABA, MDA, SOD, soluble glucose and proline were also measured at each time points. After treated with ABA, no significant changed trends were identified in the gene expression levels at 4°C in both DNDM1 and JM22. However, significantly increased variation trends were observed in the expression of MYB80, Wrabi5 and Wrab17 after treated with ABA at 4, 0 and -10°C in DNDM. The expression of CBF1VD-22D was also upregulated after treated with ABA before 0°C in DNDM1. But no significant changed trends were identified in the genes expression in JM22 both in the control and ABA treated groups at 4, 0 and -10°C. Wcs120 were significant increased at 4, 0 and -10°C, but no significant difference was identified between the control and ABA treated groups. SOD and soluble glucose were obviously accumulated after treated with ABA outdoor in DNDM1. ABA could promote the adaptation of DNDM under LT condition via increasing the expression of MYB80, Wrabi5 and Wrab17 and accumulations of SOD and soluble glucose.
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