PJB-2017-819
APPLICATION OF SSR MARKERS FOR GENETIC DIVERSITY AND YIELD COMPONENTS IN SUGARCANE GENOTYPES
Syed Tariq Shah, Ph.D
Abstract
To evaluate agronomic, genetic and its correlation with sugarcane genotypes for diversity study, SSR markers is a valuable tool in sugarcane breeding programs. In present study, variation in 30 selected sugarcane genotypes were recorded for internodes/plant, internodes length, stalk/plant, cane thickness, plant height, sugar recovery, cane yield and chlorophyll range. In agronomic data of sugarcane genotypes maximum yield (94.00 tonnes/ha) in CPSG1550, highest recovery (13.58%) in genotype CPSG3453 and maximum chlorophyll value (32.63%) were recorded in line CP77/400. However, in molecular study a total of 16 SSR markers were used for diversity and a total of 152 bands identified in which 116 polymorphic bands with a range of 33 - 100%. Primer STMS34 amplified bands (150, 200 and 300bp), UGMS39 (150bp) and UGMS118 (300bp) in high sugar genotypes. Furthermore, for red rot studies the primer SMC31CUQ and SMC597CS showed tolerance in genotypes at 600/300 bp and high tolerance at 200/600 bp banding pattern respectively. In this study, 8 % PAGE gel was standardised first time for obvious and clear banding pattern with modified silver staining method for sugarcane diversity/traits. The dendrogram result showed two clusters with 13 genotypes in cluster I and 17 genotypes in cluster II. However, analysis of correlation such as in S. officinarum genotype high rate of yield CPSG1550, maximum sugar recovery percentage CPSG3453 and high level of the chlorophyll value CP77/400 found in group III in cluster II of the dendrogram. All these data strongly interconnected and need further research for variety development in short time.
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