PJB-2017-828
Molecular Characterization of Strawberry Germplasm
Ayesha Zafar
Abstract
This study was initiated to assess the genetic diversity of strawberry in Northwest Pakistan. Since its introduction to agroclimate of Pakistan, strawberry has attained a high value cash crop for small scale farmers. Since the main cultivar across fields in Northwest Pakistan is Chandler. The assessment of genetic diversity using SSR markers in a total of four strawberry (Fragaria) varieties namely Mark, Chandler, Camino Real and Tuft; and development of diagnostic markers for identification of each variety. Among these, Chandler is the main cultivar, Tuft is grown in some parts of Mardan and Charsadda districts, while Mark and Camino Real are new introductions from Australia and USA, respectively. Among 12 SSR primers used, variable banding patterns of amplified alleles in the range of 200 bps to 400 bps were observed. For Pakistani adapted varieties, i.e., Chandler and Tuft, Marker EMFvi-109 is a better differential marker for identification. Marker EMF vi-166 was suitable for differentiating United States variety i.e., Camino Real from the rest of three cultivars. Mark, an Australian variety was differentiated from the rest of cultivars by primer EMFvi-002, 008, 018 and 025. Four Markers named EMFvi- 072, 146, 108 and 179 showed no polymorphism in all the four varieties. Among all the four varieties, Camino Real and Chandler were closely related to each other and Tuft stands a little distant from them. Moreover, Mark is genetically distant from other US varieties. Here, we report detailed information on commercially grown strawberry varieties from two different origins using SSR markers. These results could be used for broadening the genetic base of commercially grown varieties and for nursery certification by relevant seed certification department.
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