PJB-2017-830
GENETIC TRANSFORMATION OF BALANITES AEGYPTIACA USING AGROBACTERIUM TUMEFACIENS
GALAL KHAMIS
Abstract
Balanites aegyptiaca,belonging to family Zygophyllaceae, is multipurpose tree considered highly drought-tolerant, but salt-sensitive, and cultivated in several arid and tropical regions in North and West Africa and West Asia. The plant parts contain several secondary metabolites, and there is high oil content in the kernels already used in biodiesel production. This study was aimed at investigating genetic transformation between two B. aegyptiacagenotypes collected from two locations (Halayeb, Egypt and Riyadh, Saudi Arabia) using Agrobacterium tumefaciens strain GV3101 harboring the binary vector pBinAR containing ERD10 (EARLY RESPONSIVE TO DEHYDRATION 10) and nptII genes, to produce salt-tolerant B. aegyptiaca plants. The results showed that each genotype collected from the different geographical locations after transformation exhibited significant differences in the number of leaves per regenerated explant and shoot length, where by nodal explants from Halayeb, Egypt exhibited the highest values. The presence of both ERD10 and nptII genes was confirmed only in the transformed plants from Halayeb through PCR analysis, whereas the transformed plants from Riyadh, Saudi Arabia showed negative results for the presence of both genes.
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