PJB-2017-305
PRIMING SEEDS WITH PHYTOHORMONES ALLEVIATES CADMIUM TOXICITY IN MUNG BEAN (VIGNA RADIATA L. WILCZEK) SEEDLINGS
Meher Hassan and Simeen Mansoor
Abstract
Cadmium toxicity has deleterious effects on human, animals and plants. Mung bean seeds were sown in petri dishes after imbibing them in 50 µM Salicylic acid (SA), 100 µM Gibberellic acid (GA3) and distilled water (D/W) for 12 h and allowed to grow for 24h at 30°C. Seedlings were then treated with cadmium (0.3 mM and 0.5 mM) and harvested at 96h. Results revealed a significant decrease in all growth parameters and low level of protein in Cd treated seedlings as compared to control. In contrast, elevated levels of MDA, H2O2, antioxidant enzymes, tannin and proline were observed in cadmium treated seedlings. However, pretreatment with SA and GA3 prior to cadmium stress showed improvement in all growth parameters and protein content with the significant decline in MDA, H2O2, tannin and proline under cadmium stress that confers tolerance. Antioxidant enzymes seemed to help in declining H2O2 and MDA contents that confer membrane stability. Conclusively these results confirm the ameliorating effects of SA and GA3 under cadmium toxicity. As few articles are available on effect of mung bean under cadmium stress, therefore our research will have some contribution in understanding the damaging effect of cadmium and its amelioration by phytohormones in mung bean. We found that pretreatment of GA3 and SA is an effective way to enhance tolerance against cadmium in mung bean. Furthermore, the response of GA3 was more effective than SA under cadmium stress.
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