PJB-2017-323
Investigation and confirmation of introgression in Brassica species using microsatellite markers
Sidra Iqbal, Farhatullah, Malik Ashiq Rabbani, Mahwish Kanwal, Nahida Yousaf and Sania Begum
Abstract
Microsatellite markers or simple sequence repeats (SSR) are believed to be a reliable tool in the assessment of genetic variation within genomes and are therefore, widely used in population studies. In the present study, microsatellite markers were employed to confirm the process of introgression in F2hybrids derived from interspecific crosses of Oleiferous brassica species viz., B. napus (AACC genome- used as female parent) crossed with each B. juncea (AABB) and B. rapa (AA). A total of 31 SSR primers were utilized and 99 alleles were detected, with 69 (69.7%) being polymorphic. Polymorphism information content of the primer sets ranged from 0.24 to 0.75. In population of B. napus x B. junceae, pair wise similarity indices ranged from 4.4 to 9.2and from 4.2 to 8.2 in F2 hybrids ofB. napus x B. rapa. The deviation of Introgressed hybrids towards female parent in both populations revealed that C genome has played a vital role in the introgression process of brassica species under study. Our results stressed upon the use of SSR markers in assessment of genetic diversity at molecular level.
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